Chester crashed out of the FA Cup at the Fourth Qualifying Round stage following a 1-0 defeat to Southport at Haig Avenue.
Declan Weeks' first half deflected effort separated the two sides despite a lacklustre game for entertainment on the Merseyside coast.
The Blues went into the game as the form side - unbeaten in seven games with seven clean sheets to boast also. Whereas the Sandgrounders found themselves bottom of the table and with only one victory in the last six.
Despite early pressure from Chester, the home side looked the more frightening going forward - with Jamie Allen finding space in behind the Blues defence to narrowly fire over the bar from range.
Ex-Blues boss Steve Burr was evidently eager for his side to make their mark on the game after such a positive start.
And, on the 700 minute mark since last conceding a goal, the Blues fell behind through a deflected strike from midfielder Declan Weeks. The 20 year-old picked up a sliced clearance to fire at Liam Nolan from 25 yards out - with the ricochet leaving Roberts rooted to the spot.
Neither side looked like stamping their authority on the second half - but the Blues were looking ever more likely to sneak a equaliser.
Craig Mahon impressed on the left flank throughout the encounter - weaving past ex-Blue Higgins to curl in a cross for Alabi, who narrowly headed wide of Norman's far post.
The Irishman also provided many moments late on to push forward. After a magnificent spell of possession on the left side, Jordan Chapell guided his strike from the edge of the area onto the finger tips of Sandgrounders keeper Magnus Norman - much to the disgruntlement of the travelling Blues supporters behind the goal.
Chester couldn't find the equaliser to save their FA Cup ambitions - and Southport will now enter the draw on Monday evening as ball number 49.
Chester: Roberts, Vassell, Hughes, Astles, Hunt (Horwood 82), Mahon, George, Lloyd, Durrell (Chapell 29), Richards (Akintunde 60), Alabi.
Subs not used: Evans, Waters, Killock, Joyce
Referee: Wayne Barratt
Attendance: 1674 (738 away)