An early Danny Carlton goal ensured Chester kept their fight against relegation in their own hands going into the final day with a narrow victory over second placed Cambridge.
The result means the blues are two points clear of the drop zone going into their crucial clash at home to Salisbury on Saturday (3pm).
A delighted Steve Burr made just one change from the goalless draw in the Cross-Border Derby on Saturday, as Jamie Menagh came in for the injured Gareth Seddon.
On-loan Hyde striker Carlton gave Chester a 12th minute lead as he headed home from close range after a good clipped delivery by Lewis Turner.
The blues were dent a huge blow a matter of minutes later when George Horan went off injured for the second consecutive game, replaced by Luke Ashworth.
Moments later Cambridge striker Tom Elliot fired wide after good work by Andy Pugh, but the Chester backline restricted the hosts chances to a minimum and finished the half a goal up.
The visitors were then to threaten on numerous occasions in the second half. Danny Carlton headed wide and then had a deflected shot saved when he broke the offside trp, before Andy Bond missed a
glorious one-on-one after being set up by John Rooney.
Cambridge sub Matthew Barnes-Homer had some glorious chances to equalise but the Chester defence continued to hold solid, before Aaron Chapman made a vital save after being rounded up by Ashley Chambers - to ensure the struggling visitors picked up a crucial three points.
Cambridge United: Norris, Tait (Chambers 58), Miller, Coulson, Taylor, Hughes, Champion, Berry
(Dunk 46), Chadwick, Elliott (Barnes-Homer 58), Pugh.
Subs Not Used: Roberts, Bonner.
Chester FC: Chapman, Griffin (Mahon 68), Horan (Ashworth 26), Linwood, McIntyre, Turner, Bond, Jarrett, Menagh, Rooney, Carlton (Hobson 82).
Subs Not Used: Danby, Lindfield.
Goal: Carlton 12.
Booked: Linwood, Jarrett, McIntyre.
Referee: Jason Whiteley (Leeds)