League Champions, Chester FC went down to their first league defeat in 31 games after they were beaten 2-1 by Guiseley, the team who have been the Blues' closest promotion rivals in the second half of the season.
Josh Wilson netted both of the host's goals whilst Tony Gray scored for Chester just before half time.
Chester made an attacking start to the match in the 2nd minute, Matty McGinn's ball being met by George Horan but his header was saved by Steve Drench in the Guiseley goal, diving to his right to make a good save.
The hosts took the lead in the 7th minute, Rothery was felled on the right edge of the area, and Josh Wilson's free kick beat John Danby to his left, whose sight was possibly hindered by the wall0.
Chester, in their familiar green and black away kit almost struck back on 14 minutes, Matty McGinn with a free kick who curled the ball around the wall but was well saved.
Guiseley had an opportunity to increase their lead in the 18th minute, after Holdsworth was clipped by Tony Gray, but the free kick from the left side this time, a bit further out, curled round the wall and hit the post.
Antoni Sarcevic almost pulled the Blues level on 36 minutes, after Nathan Jarman found the attacking midfielder on the edge of the area, but his shot was low but wide.
Again, Chester came close in the 38th minute, Tony Gray got one on one with Drench but the in-form striker could only fire wide.
Then just into first half added time, Sarcevic delivered a corner which Tony Gray appeared to head into the top corner, strangely there were muted celebrations which hinted that there was possibly a claim of an own goal, but the Blues were level going into the half time break.
Away fans were expecting the Champions to get a better grip of the game having clawed their way back into it, but on 57 minutes, Josh Wilson played Holsgrave through, who seemed destined to put Guiseley 2-1 up but Dom Collins made a stunning last ditch challenge, inside the area to stop the shot.
Chester then started to attack the Lions again, on 58 minutes, Sarcevic shot from ten yards out, whilst backing away from goal but saw his effort go wide, then perhaps the best chance of all for Sarcevic as he found himself through on goal after a deft flick from Jarman, but Drench came out on top again as Sarcevic's effort went straight at him.
Just a minute later and again Drench was there to prevent Chester from taking the lead, when he denied Sarcevic from close range again, and the Champions were made to pay for it just 2 minutes later.
Matty McGinn failed to cut out a ball to Josh Wilson who then found himself one on one with Danby before lifting it over the Chester keeper to put the home side 2-1 up.
With the unbeaten league record now under threat, Chester tried to find an equalizer, and on 77 minutes, Dave Hankin crossed to Tony Gray, who hit it goalwards, but Danny Williams could only head over from close range.
On 83 minutes, former Blues player Jack Rea had a run and shot just wide of the far post, and will maybe think he should have done better.
Chester's final effort of the game was in the third minute of stoppage time when McGinn delivered a corner that John Danby raced upfield for, and got his head to it to find Danny Williams, but once again found Drench in inspired form, who saved the last ditch effort and the ball was scrambled away before the referee signalled the end of the match, and with it the end of Chester's 30 match unbeaten league record, falling just one short of the Club record set over 100 years ago.
Guiseley: Drench, Hardy, Meynell, Rea, Ellis, M Wilson, J Wilson, Holdsworth (c), Holsgrave (Walshaw 63), Carole, Rothery
Subs: McWilliams, Bower, Mullen, Gilles
Chester: Danby, L Turner, McGinn, Horan (c)(Baynes 45), Collins, N Turner (M Williams 88), Hankin, Sarcevic, Jarman, Gray (A Williams 77), D Williams
Subs: Howard, Brown
Goal Scorers: Chester: Gray (45+1). Guiseley: Josh Wilson (7,70).
Referee: Mr M Bristow
Assistant Referees: Mr A Cawtherley, Mr T Kirk